Keith Neeves, PhD
Professor, University of Colorado - Denver

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Graduate School :
  • PhD, Cornell University (2006)
Undergraduate School:
  • BS, University of Colorado - Boulder (CO) (2000)
Department: University of Colorado - Denver

Research Interests

Hemostasis, thrombosis, hemodynamics, bleeding disorders, stroke, drug delivery, diagnostics

Publications

  • Link KG, Stobb MT, Sorrells MG, Bortot M, Ruegg K, Manco-Johnson MJ, Di Paola JA, Sindi SS, Fogelson AL, Leiderman K, Neeves KB. A mathematical model of coagulation under flow identifies factor V as a modifier of thrombin generation in hemophilia A. J Thromb Haemost. 2020 Feb;18(2):306-317. PubMed PMID: 31562694
  • Davizon-Castillo P, Allawzi A, Sorrells M, Fisher S, Baltrunaite K, Neeves K, Nozik-Grayck E, DiPaola J, Delaney C. Platelet activation in experimental murine neonatal pulmonary hypertension. Physiol Rep. 2020 Mar;8(5):e14386. PubMed PMID: 32163236
  • Bortot M, Ashworth K, Sharifi A, Walker F, Crawford NC, Neeves KB, Bark D Jr, Di Paola J. Turbulent Flow Promotes Cleavage of VWF (von Willebrand Factor) by ADAMTS13 (A Disintegrin and Metalloproteinase With a Thrombospondin Type-1 Motif, Member 13). Arterioscler Thromb Vasc Biol. 2019 Sep;39(9):1831-1842. PubMed PMID: 31291760
  • Moore HB, Neeves KB. Tranexamic acid for trauma: Repackaged and redelivered. J Thromb Haemost. 2019 Oct;17(10):1626-1628. PubMed PMID: 31571419
  • Disharoon D, Neeves KB, Marr DWM. ac/dc Magnetic Fields for Enhanced Translation of Colloidal Microwheels. Langmuir. 2019 Mar 5;35(9):3455-3460. PubMed PMID: 30726100
  • Yang T, Tomaka A, Tasci TO, Neeves KB, Wu N, Marr DWM. Microwheels on Microroads: Enhanced Translation on Topographic Surfaces. Sci Robot. 2019 Jul 31;4(32). PubMed PMID: 31592128
  • Disharoon D, Marr DWM, Neeves KB. Engineered microparticles and nanoparticles for fibrinolysis. J Thromb Haemost. 2019 Dec;17(12):2004-2015. PubMed PMID: 31529593
  • Lehmann M, Schoeman RM, Krohl PJ, Wallbank AM, Samaniuk JR, Jandrot-Perrus M, Neeves KB. Platelets Drive Thrombus Propagation in a Hematocrit and Glycoprotein VI-Dependent Manner in an In Vitro Venous Thrombosis Model. Arterioscler Thromb Vasc Biol. 2018 May;38(5):1052-1062. PubMed PMID: 29472230
  • Lehmann M, Ashworth K, Manco-Johnson M, Di Paola J, Neeves KB, Ng CJ. Evaluation of a microfluidic flow assay to screen for von Willebrand disease and low von Willebrand factor levels. J Thromb Haemost. 2018 Jan;16(1):104-115. PubMed PMID: 29064615
  • Branchford BR, Stalker TJ, Law L, Acevedo G, Sather S, Brzezinski C, Wilson KM, Minson K, Lee-Sherick AB, Davizon-Castillo P, Ng C, Zhang W, Neeves KB, Lentz SR, Wang X, Frye SV, Shelton Earp H 3rd, DeRyckere D, Brass LF, Graham DK, Di Paola JA. The small-molecule MERTK inhibitor UNC2025 decreases platelet activation and prevents thrombosis. J Thromb Haemost. 2018 Feb;16(2):352-363. PubMed PMID: 29045015
  • Link KG, Stobb MT, Di Paola J, Neeves KB, Fogelson AL, Sindi SS, Leiderman K. A local and global sensitivity analysis of a mathematical model of coagulation and platelet deposition under flow. PLoS One. 2018;13(7):e0200917. PubMed PMID: 30048479
  • Rod K, Um W, Chun J, Wu N, Yin X, Wang G, Neeves K. Effect of chemical and physical heterogeneities on colloid-facilitated cesium transport. J Contam Hydrol. 2018 Jun;213:22-27. PubMed PMID: 29657081
  • Tasci TO, Disharoon D, Schoeman RM, Rana K, Herson PS, Marr DWM, Neeves KB. Enhanced Fibrinolysis with Magnetically Powered Colloidal Microwheels. Small. 2017 Sep;13(36). PubMed PMID: 28719063
  • Walton BL, Lehmann M, Skorczewski T, Holle LA, Beckman JD, Cribb JA, Mooberry MJ, Wufsus AR, Cooley BC, Homeister JW, Pawlinski R, Falvo MR, Key NS, Fogelson AL, Neeves KB, Wolberg AS. Elevated hematocrit enhances platelet accumulation following vascular injury. Blood. 2017 May 4;129(18):2537-2546. PubMed PMID: 28251913
  • Naik MU, Patel P, Derstine R, Turaga R, Chen X, Golla K, Neeves KB, Ichijo H, Naik UP. Ask1 regulates murine platelet granule secretion, thromboxane A(2) generation, and thrombus formation. Blood. 2017 Mar 2;129(9):1197-1209. PubMed PMID: 28028021
  • Yang T, Tasci TO, Neeves KB, Wu N, Marr DWM. Magnetic Microlassos for Reversible Cargo Capture, Transport, and Release. Langmuir. 2017 Jun 13;33(23):5932-5937. PubMed PMID: 28318267
  • Schoeman RM, Lehmann M, Neeves KB. Flow chamber and microfluidic approaches for measuring thrombus formation in genetic bleeding disorders. Platelets. 2017 Jul;28(5):463-471. PubMed PMID: 28532218
  • Schoeman RM, Rana K, Danes N, Lehmann M, Di Paola JA, Fogelson AL, Leiderman K, Neeves KB. A microfluidic model of hemostasis sensitive to platelet function and coagulation. Cell Mol Bioeng. 2017 Feb;10(1):3-15. PubMed PMID: 28529666
  • Cooper S, Lloyd S, Koch A, Lin X, Dobbs K, Theisen T, Zuberbuehler M, Bernhardt K, Gyorfi M, Tenpas T, Hying S, Mortimer S, Lamont C, Lehmann M, Neeves K. Temperature effects on the activity, shape, and storage of platelets from 13-lined ground squirrels. J Comp Physiol B. 2017 Jul;187(5-6):815-825. PubMed PMID: 28332020
  • Guo Y, Huang J, Xiao F, Yin X, Chun J, Um W, Neeves KB, Wu N. Bead-Based Microfluidic Sediment Analogues: Fabrication and Colloid Transport. Langmuir. 2016 Sep 13;32(36):9342-50. PubMed PMID: 27548505
  • Rana K, Neeves KB. Blood flow and mass transfer regulation of coagulation. Blood Rev. 2016 Sep;30(5):357-68. PubMed PMID: 27133256
  • Maier FJ, Lachner T, Vilfan A, Tasci TO, Neeves KB, Marr DW, Fischer TM. Non reciprocal skewed rolling of a colloidal wheel due to induced chirality. Soft Matter. 2016 Nov 23;12(46):9314-9320. PubMed PMID: 27824198
  • McCarty OJ, Ku D, Sugimoto M, King MR, Cosemans JM, Neeves KB. Dimensional analysis and scaling relevant to flow models of thrombus formation: communication from the SSC of the ISTH. J Thromb Haemost. 2016 Mar;14(3):619-22. PubMed PMID: 26933837
  • Roth KB, Neeves KB, Squier J, Marr DW. High-throughput linear optical stretcher for mechanical characterization of blood cells. Cytometry A. 2016 Apr;89(4):391-7. PubMed PMID: 26565892
  • Lehmann M, Wallbank AM, Dennis KA, Wufsus AR, Davis KM, Rana K, Neeves KB. On-chip recalcification of citrated whole blood using a microfluidic herringbone mixer. Biomicrofluidics. 2015 Nov;9(6):064106. PubMed PMID: 26634014
  • Roth KB, Neeves KB, Squier J, Marr DW. Imaging of a linear diode bar for an optical cell stretcher. Biomed Opt Express. 2015 Mar 1;6(3):807-14. PubMed PMID: 25798305
  • Neeves KB. Down in a hole: a new laser ablation model of hemostasis. J Thromb Haemost. 2015 Mar;13(3):414-6. PubMed PMID: 25510738
  • Sylman JL, Artzer DT, Rana K, Neeves KB. A vascular injury model using focal heat-induced activation of endothelial cells. Integr Biol (Camb). 2015 Jul;7(7):801-14. PubMed PMID: 26087748
  • Branchford BR, Ng CJ, Neeves KB, Di Paola J. Microfluidic technology as an emerging clinical tool to evaluate thrombosis and hemostasis. Thromb Res. 2015 Jul;136(1):13-9. PubMed PMID: 26014643
  • Wufsus AR, Rana K, Brown A, Dorgan JR, Liberatore MW, Neeves KB. Elastic behavior and platelet retraction in low- and high-density fibrin gels. Biophys J. 2015 Jan 6;108(1):173-83. PubMed PMID: 25564864
  • Fogelson AL, Neeves KB. Fluid Mechanics of Blood Clot Formation. Annu Rev Fluid Mech. 2015 Jan 1;47:377-403. PubMed PMID: 26236058
  • Neeves KB. Physiochemical artifacts in FeCl3 thrombosis models. Blood. 2015 Aug 6;126(6):700-1. PubMed PMID: 26251225
  • Zhu S, Herbig BA, Li R, Colace TV, Muthard RW, Neeves KB, Diamond SL. In microfluidico: Recreating in vivo hemodynamics using miniaturized devices. Biorheology. 2015;52(5-6):303-18. PubMed PMID: 26600269
  • Thon JN, Mazutis L, Wu S, Sylman JL, Ehrlicher A, Machlus KR, Feng Q, Lu S, Lanza R, Neeves KB, Weitz DA, Italiano JE Jr. Platelet bioreactor-on-a-chip. Blood. 2014 Sep 18;124(12):1857-67. PubMed PMID: 25606631
  • Rana K, Timmer BJ, Neeves KB. A combined microfluidic-microstencil method for patterning biomolecules and cells. Biomicrofluidics. 2014 Sep;8(5):056502. PubMed PMID: 25332748
  • Onasoga-Jarvis AA, Puls TJ, O'Brien SK, Kuang L, Liang HJ, Neeves KB. Thrombin generation and fibrin formation under flow on biomimetic tissue factor-rich surfaces. J Thromb Haemost. 2014;12(3):373-82. PubMed PMID: 24345079
  • Neeves KB, McCarty OJ, Reininger AJ, Sugimoto M, King MR. Flow-dependent thrombin and fibrin generation in vitro: opportunities for standardization: communication from SSC of the ISTH. J Thromb Haemost. 2014;12(3):418-20. PubMed PMID: 24330648
  • Neeves KB, Onasoga AA, Wufsus AR. The use of microfluidics in hemostasis: clinical diagnostics and biomimetic models of vascular injury. Curr Opin Hematol. 2013 Sep;20(5):417-23. PubMed PMID: 23872531
  • Sylman JL, Lantvit SM, Vedepo MC, Reynolds MM, Neeves KB. Transport limitations of nitric oxide inhibition of platelet aggregation under flow. Ann Biomed Eng. 2013 Oct;41(10):2193-205. PubMed PMID: 23563992
  • Wu Q, Ok JT, Sun Y, Retterer ST, Neeves KB, Yin X, Bai B, Ma Y. Optic imaging of single and two-phase pressure-driven flows in nano-scale channels. Lab Chip. 2013 Mar 21;13(6):1165-71. PubMed PMID: 23370894
  • Neeves KB. Collagen-induced thrombosis in large vessels using native prothrombotic substrates. Thromb Res. 2013 Jan;131(1):1-2. PubMed PMID: 23174625
  • Ahmad S, Ahmad A, Rancourt RC, Neeves KB, Loader JE, Hendry-Hofer T, Di Paola J, Reynolds SD, White CW. Tissue factor signals airway epithelial basal cell survival via coagulation and protease-activated receptor isoforms 1 and 2. Am J Respir Cell Mol Biol. 2013 Jan;48(1):94-104. PubMed PMID: 23065128
  • Hansen RR, Wufsus AR, Barton ST, Onasoga AA, Johnson-Paben RM, Neeves KB. High content evaluation of shear dependent platelet function in a microfluidic flow assay. Ann Biomed Eng. 2013 Feb;41(2):250-62. PubMed PMID: 23001359
  • Roth KB, Eggleton CD, Neeves KB, Marr DW. Measuring cell mechanics by optical alignment compression cytometry. Lab Chip. 2013 Apr 21;13(8):1571-7. PubMed PMID: 23440063
  • Wufsus AR, Macera NE, Neeves KB. The hydraulic permeability of blood clots as a function of fibrin and platelet density. Biophys J. 2013 Apr 16;104(8):1812-23. PubMed PMID: 23601328
  • Onasoga-Jarvis AA, Leiderman K, Fogelson AL, Wang M, Manco-Johnson MJ, Di Paola JA, Neeves KB. The effect of factor VIII deficiencies and replacement and bypass therapies on thrombus formation under venous flow conditions in microfluidic and computational models. PLoS One. 2013;8(11):e78732. PubMed PMID: 24236042
  • Neeves KB, Onasoga AA, Hansen RR, Lilly JJ, Venckunaite D, Sumner MB, Irish AT, Brodsky G, Manco-Johnson MJ, Di Paola JA. Sources of variability in platelet accumulation on type 1 fibrillar collagen in microfluidic flow assays. PLoS One. 2013;8(1):e54680. PubMed PMID: 23355889
  • Neeves KB. Catch me if you can: isolating circulating tumor cells from flowing blood. Clin Chem. 2012 May;58(5):803-4. PubMed PMID: 22353214
  • Gupta VK, Neeves KB, Eggleton CD. Effect of viscoelasticity on the analysis of single-molecule force spectroscopy on live cells. Biophys J. 2012 Jul 3;103(1):137-45. PubMed PMID: 22828340
  • Wu M, Xiao F, Johnson-Paben RM, Retterer ST, Yin X, Neeves KB. Single- and two-phase flow in microfluidic porous media analogs based on Voronoi tessellation. Lab Chip. 2012 Jan 21;12(2):253-61. PubMed PMID: 22094719
  • Foley CP, Nishimura N, Neeves KB, Schaffer CB, Olbricht WL. Real-time imaging of perivascular transport of nanoparticles during convection-enhanced delivery in the rat cortex. Ann Biomed Eng. 2012 Feb;40(2):292-303. PubMed PMID: 22009318
  • Ahmad S, Raemy DO, Loader JE, Kailey JM, Neeves KB, White CW, Ahmad A, Gehr P, Rothen-Rutishauser BM. Interaction and localization of synthetic nanoparticles in healthy and cystic fibrosis airway epithelial cells: effect of ozone exposure. J Aerosol Med Pulm Drug Deliv. 2012 Feb;25(1):7-15. PubMed PMID: 22007674
  • Hansen RR, Tipnis AA, White-Adams TC, Di Paola JA, Neeves KB. Characterization of collagen thin films for von Willebrand factor binding and platelet adhesion. Langmuir. 2011 Nov 15;27(22):13648-58. PubMed PMID: 21967679
  • Olbricht WL, Neeves KB, Foley CP. Microfluidic probes in the treatment of brain-related diseases. Drug News Perspect. 2010 Oct;23(8):491-7. PubMed PMID: 21031165
  • Wannemacher KM, Zhu L, Jiang H, Fong KP, Stalker TJ, Lee D, Tran AN, Neeves KB, Maloney S, Kumanogoh A, Kikutani H, Hammer DA, Diamond SL, Brass LF. Diminished contact-dependent reinforcement of Syk activation underlies impaired thrombus growth in mice lacking Semaphorin 4D. Blood. 2010 Dec 16;116(25):5707-15. PubMed PMID: 20855865
  • Neeves KB, Illing DA, Diamond SL. Thrombin flux and wall shear rate regulate fibrin fiber deposition state during polymerization under flow. Biophys J. 2010 Apr 7;98(7):1344-52. PubMed PMID: 20371335
  • Zhu L, Stalker TJ, Fong KP, Jiang H, Tran A, Crichton I, Lee EK, Neeves KB, Maloney SF, Kikutani H, Kumanogoh A, Pure E, Diamond SL, Brass LF. Disruption of SEMA4D ameliorates platelet hypersensitivity in dyslipidemia and confers protection against the development of atherosclerosis. Arterioscler Thromb Vasc Biol. 2009 Jul;29(7):1039-45. PubMed PMID: 19390055
  • Schmaier AA, Zou Z, Kazlauskas A, Emert-Sedlak L, Fong KP, Neeves KB, Maloney SF, Diamond SL, Kunapuli SP, Ware J, Brass LF, Smithgall TE, Saksela K, Kahn ML. Molecular priming of Lyn by GPVI enables an immune receptor to adopt a hemostatic role. Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21167-72. PubMed PMID: 19940238
  • Foley CP, Nishimura N, Neeves KB, Schaffer CB, Olbricht WL. Flexible microfluidic devices supported by biodegradable insertion scaffolds for convection-enhanced neural drug delivery. Biomed Microdevices. 2009 Aug;11(4):915-24. PubMed PMID: 19353271
  • Neeves KB, Diamond SL. A membrane-based microfluidic device for controlling the flux of platelet agonists into flowing blood. Lab Chip. 2008 May;8(5):701-9. PubMed PMID: 18432339
  • Neeves KB, Maloney SF, Fong KP, Schmaier AA, Kahn ML, Brass LF, Diamond SL. Microfluidic focal thrombosis model for measuring murine platelet deposition and stability: PAR4 signaling enhances shear-resistance of platelet aggregates. J Thromb Haemost. 2008 Dec;6(12):2193-201. PubMed PMID: 18983510
  • Okorie UM, Denney WS, Chatterjee MS, Neeves KB, Diamond SL. Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow. Blood. 2008 Apr 1;111(7):3507-13. PubMed PMID: 18203955
  • Neeves KB, Sawyer AJ, Foley CP, Saltzman WM, Olbricht WL. Dilation and degradation of the brain extracellular matrix enhances penetration of infused polymer nanoparticles. Brain Res. 2007 Nov 14;1180:121-32. PubMed PMID: 17920047
  • Spence AJ, Neeves KB, Murphy D, Sponberg S, Land BR, Hoy RR, Isaacson MS. Flexible multielectrodes can resolve multiple muscles in an insect appendage. J Neurosci Methods. 2007 Jan 15;159(1):116-24. PubMed PMID: 16899299
  • Neeves KB, Lo CT, Foley CP, Saltzman WM, Olbricht WL. Fabrication and characterization of microfluidic probes for convection enhanced drug delivery. J Control Release. 2006 Apr 10;111(3):252-62. PubMed PMID: 16476500
  • Retterer ST, Smith KL, Bjornsson CS, Neeves KB, Spence AJ, Turner JN, Shain W, Isaacson MS. Model neural prostheses with integrated microfluidics: a potential intervention strategy for controlling reactive cell and tissue responses. IEEE Trans Biomed Eng. 2004 Nov;51(11):2063-73. PubMed PMID: 15536908
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Professional Memberships

  • American Heart Association, Fellow
  • American Society of Hematology, Member
  • Biomedical Engineering Society, Member
  • International Society on Thrombosis and Haemostasis, Member

General Information

Graduate Schools:
  • PhD, Cornell University (2006)
Undergraduate Schools:
  • BS, University of Colorado - Boulder (CO) (2000)
Department: University of Colorado - Denver
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