Heidi Chial, PhD
Assistant Research Professor, Neurology

Photo
Download CV
Graduate School :
  • PhD, University of Colorado - Boulder (1998)
Undergraduate School:
  • BA, Gustavus Adolphus College (MN) (1993)
Fellowships:
  • College of Medicine, Mayo Clinic (Rochester) Program, Postdoctoral Research Fellowship, Dept. Biochemistry and Molecular Biology, Cancer Biology (1999)
  • Wake Forest University School of Medicine Program, Postdoctoral Research Fellowship, Dept. Cancer Biology (2006)
  • Stanford University Program, Postdoctoral Research Fellowship, Dept. Neurology and Neurological Sciences and the Neuroscience Institute at Stanford (2008)
Department: Neurology

Professional Titles

  • Director of Grant Strategy and Development, University of Colorado Alzheimer's and Cognition Center

Research Interests

I have extensive experience and a long-standing interest in using cell biological and genetic approaches to uncover mechanistic links between chromosomal aneuploidy and human disease. My studies of aneuploidy began as a graduate student in Dr. Mark Winey’s laboratory at the University of Colorado-Boulder, where I discovered a shared localization of yeast Ndc1p to spindle poles and nuclear pore complexes and I identified NDC1 gene dosage effects — both haploinsufficiency and overexpression — that lead to genetic instability and aneuploidy in yeast. Based on these findings, we hypothesized that the phenotypes associated with altered NDC1 gene dosage and its ability to induce aneuploidy could serve as a model for understanding human diseases associated with genetic instability, including cancer and neurodegenerative disease. In my postdoctoral training, I worked in cancer biology labs at the Mayo Clinic College of Medicine and Wake Forest University School of Medicine, and then pursued additional training in molecular and cellular neuroscience at the Marine Biological Laboratory and at Stanford University School of Medicine. At Stanford University, my research was aimed at understanding the role of a pair of RAB5 effectors — called APPL1 and APPL2 — in neurotrophin- and endosome-mediated signaling pathways in neurodegenerative disease with a special focus on the connections between Alzheimer’s disease and Down syndrome/Trisomy 21. Since joining Dr. Potter’s research group at the University of Colorado Alzheimer's and Cognition Center in 2015, I have been involved in the design, implementation, analysis, and publication of research projects focused on the connections between aneuploidy, Alzheimer’s disease, and other forms of neurodegenerative disease, such as frontotemporal lobar degeneration (FTLD) and Huntington’s disease, links between Alzheimer’s disease and Down syndrome, and developing novel Alzheimer’s disease therapeutics.

Publications

  • Coughlan C, Bruce KD, Burgy O, Boyd TD, Michel CR, Garcia-Perez JE, Adame V, Anton P, Bettcher BM, Chial HJ, Königshoff M, Hsieh EWY, Graner M, Potter H. Exosome Isolation by Ultracentrifugation and Precipitation and Techniques for Downstream Analyses. Curr Protoc Cell Biol. 2020 Sep;88(1):e110. PubMed PMID: 32633898
  • Potter H, Chial HJ, Caneus J, Elos M, Elder N, Borysov S, Granic A. Chromosome Instability and Mosaic Aneuploidy in Neurodegenerative and Neurodevelopmental Disorders. Front Genet. 2019;10:1092. PubMed PMID: 31788001
  • Potter H, Chial HJ. Targeting the Interaction Between Apolipoprotein E and Amyloid Precursor Protein: A Novel Alzheimer's Disease Therapy. Biol Psychiatry. 2019 Aug 1;86(3):169-170. PubMed PMID: 31319935
  • Hamlett ED, Ledreux A, Potter H, Chial HJ, Patterson D, Espinosa JM, Bettcher BM, Granholm AC. Exosomal biomarkers in Down syndrome and Alzheimer's disease. Free Radic Biol Med. 2018 Jan;114:110-121. PubMed PMID: 28882786
  • Caneus J, Granic A, Rademakers R, Dickson DW, Coughlan CM, Chial HJ, Potter H. Mitotic Defects Lead to Neuronal Aneuploidy and Apoptosis in Frontotemporal Lobar Degeneration Caused by MAPT Mutations. Mol Biol Cell. 2017 Dec 27. [Epub ahead of print] PubMed PMID: 29282277
  • Chial HJ, Lenart P, Chen YQ. APPL proteins FRET at the BAR: direct observation of APPL1 and APPL2 BAR domain-mediated interactions on cell membranes using FRET microscopy. PLoS One. 2010 Aug 30;5(8):e12471. PubMed PMID: 20814572
  • Chial HJ, Wu R, Ustach CV, McPhail LC, Mobley WC, Chen YQ. Membrane targeting by APPL1 and APPL2: dynamic scaffolds that oligomerize and bind phosphoinositides. Traffic. 2008 Feb;9(2):215-29. PubMed PMID: 18034774
  • Chial HJ, Stemm-Wolf AJ, McBratney S, Winey M. Yeast Eap1p, an eIF4E-associated protein, has a separate function involving genetic stability. Curr Biol. 2000 Nov 30;10(23):1519-22. PubMed PMID: 11114520
  • Chial HJ, Winey M. Mechanisms of genetic instability revealed by analysis of yeast spindle pole body duplication. Biol Cell. 1999 Jul;91(6):439-50. PubMed PMID: 10519004
  • Chial HJ, Giddings TH Jr, Siewert EA, Hoyt MA, Winey M. Altered dosage of the Saccharomyces cerevisiae spindle pole body duplication gene, NDC1, leads to aneuploidy and polyploidy. Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10200-5. PubMed PMID: 10468586
  • Chial HJ, Rout MP, Giddings TH, Winey M. Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies. J Cell Biol. 1998 Dec 28;143(7):1789-800. PubMed PMID: 9864355
  • Chial HJ, Splittgerber AG. A comparison of the binding of Coomassie brilliant blue to proteins at low and neutral pH. Anal Biochem. 1993 Sep;213(2):362-9. PubMed PMID: 7694522
  • Chial HJ, Thompson HB, Splittgerber AG. A spectral study of the charge forms of Coomassie blue G. Anal Biochem. 1993 Mar;209(2):258-66. PubMed PMID: 7682385
  • Potter, H., Woodcock, J.H., Boyd, T.D. Coughlan, C.M., O’Shaughnessy, J.R., Borges, M.T., Thaker, A.A., Raj, B.A., Adamszuk, K., Scott, D., Adame, V., Chial., H.J., Gray, H., Daniels, J., Stocker, M.E., Sillau, S.H. Safety and Efficacy of Sargramostim in the Treatment of Alzheimer’s Disease. Alzheimer’s & Dementia: Translational Research & Clinical Interventions (2021), In Press.
View All (14 Total) View Less

General Information

Graduate Schools:
  • PhD, University of Colorado - Boulder (1998)
Undergraduate Schools:
  • BA, Gustavus Adolphus College (MN) (1993)
Fellowships:
  • College of Medicine, Mayo Clinic (Rochester) Program, Postdoctoral Research Fellowship, Dept. Biochemistry and Molecular Biology, Cancer Biology (1999)
  • Wake Forest University School of Medicine Program, Postdoctoral Research Fellowship, Dept. Cancer Biology (2006)
  • Stanford University Program, Postdoctoral Research Fellowship, Dept. Neurology and Neurological Sciences and the Neuroscience Institute at Stanford (2008)
Download CV
Department: Neurology
;